by Robert E. Farrell, Jr (1998)
This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. This edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5. and 3. RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis.
Mike Starkey, Ramnath Elaswarapyu
A team of researchers share the most current information in a field that has recently switched emphasis from gene identification to functional genomics and the characterization of genes and gene products. This volume approaches its complex subject with a broad perspective to supply its reader with a vital overview of genomics and its derivative fields, with a focus on pivotal issues such as data analysis.
[by] J. B. Harborne
Phytochemistry is a rapidly expanding area with new techniques being developed and existing ones perfected and made easier to incorporate as standard methods in the laboratory. This edition includes descriptions of methods such as HPLC and the increasingly sophisticated NMR and related spectral techniques. Other methods described are the use of NMR to locate substances within the plant cell and the chiral separation of essential oils. After an introductory chapter on methods of plant analysis, individual chapters describe methods of identifying the different type of plant molecules: phenolic compounds, terpenoids, organic acids, lipids and related compounds, nitrogen compounds, sugar and derivatives and macromolecules. Different methods are discussed and recommended, and guidance provided for the analysis of compounds of special physiological relevance such as endogenous growth regulators, substances of pharmacological interest and screening methods for the detection of substances for taxonomic purposes.
D. Rickwood and D. Patel
This volume is a compendium of essential information required by all cell and molecular biologists. As well as providing a wide range of core information needed by all researchers in the biomolecular sciences, it brings together additional selected information of importance from the areas of biochemistry, cell biology, genetics and molecular biology. This book will prove invaluable for all researchers in these fields. The Essential Data series provides rapid access to the core data required by researchers on a daily basis, in convenient pocket-sized volumes.
Robert Philip Weber
This second edition has been completely updated to include new studies, new computer applications and an additional chapter on problems and issues that can arise when carrying out content analysis in four major categories: measurement, indication, representation and interpretation.